Anna Minot's first stage role was as a chickadee in a elementary school play. By the time she graduated Phi Beta Kappa from Vassar College in 1939, she had acted in community theatre and in summer stock. She went on from there to a long and distinguished career on the stage, with occasional film and television work. She was in the first production of Eugene O'Neill's "The Iceman Cometh", where she understudied and later succeeded Jeanne Cagney; she also worked with Frederic March and Florence Eldridge. A longtime resident of Greenwich Village, for a time Miss Minot was the stage manager of the Pearl Theatre where she also acted in a myriad of productions over the years.