Luisi Pistilli's most notable stage successes were roles in "The Threepenny Opera", "St. Joan of the Stockyards" and a 1972 production, "Lulu". In 1991 he reprised his role in "Lulu" in the first professional collaboration with actress-singer Milva, his partner in previous plays as well as in a four-year offstage relationship. Pistilli's most memorable roles were in Francesco Rosi's Cadaveri eccellenti (1976), Lino Del Fra's Antonio Gramsci: i giorni del carcere (1977), Carlo Lizzani's Italo-Bulgarian co-production L'amante di Gramigna (1969) and Sergio Leone's Per qualche dollaro in più (1965) and Il buono, il brutto, il cattivo (1966), in which he played priest Pablo Ramirez, the brother of Eli Wallach's character Tuco. He also worked frequently in TV, including the Mafia series La piovra 5 - Il cuore del problema (1990), directed by Luigi Perelli.